Selection from microcarriers

When cells are growing on microcarriers ( 3.6) mitotic cells can be selectively dislodged by increasing the stirring speed (Crespi and Thilly, 1982). 

In order to produce a stirring rate of 150 r.p.m. Crespi and Thilly (1982) made a special harvesting vessel with stirring bars. Their selected cells had a viability of 80-95% and a mitotic index of 85-95%. 

This approach is unlikely to be successful with the glass or plastic beads to which cells appear to attach only poorly at all stages of the cell cycle. 

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