Secretion protein genes

Jacobs, K. A., Collins-Racie, L. A., Colbert, M., Duckett, M., Golden-Fleet, M., Kelleher, K., et al. (1997) A genetic selection for isolating cDNAs encoding secreted proteins. Gene 198, 289-296. 

The second major step of gene expression involves the translation of a protein, e.g., enzymes, structural proteins, secreted proteins, gene expression factors, etc., in which the mRNA interacts with ribosomes and amino acid activated transfer RNA to direet the synthesis of the protein coded for by the mRNA sequence. 

Schmuckli-Maurer J, Casanova C, Schmied S, Affentranger S, Parvanova I, Kang a S, Nene V, Katzer F, Keever D, Muller J, Bishop R, Pain A, Dobbelaere DA. Expression analysis of the Theilera parva subtelomeric-encoded variable secreted protein gene family. PLoS One. 2009 4(3) 4839. doi 10.1371/joumal.pone.0004839. 

The screening method based on diameter of halos yielded only five strains with at least 20% more PG than wild t> e. Most attempts to enhance pectinase production have been made in filamentous fiingi, in which enzyme production is regulated by induction and catabolite repression mechanisms [8], By contrast, PG produced by marxianus is constitutive and not subject to catabolite repression [6], Failure to find greatly enhanced secretion levels is consistent with the idea of a constitutive gene that is capable of only modest further induction. PG was found in this study to be already the most prolific secreted protein of K. marxianus. 

As is mentioned in Chapter 10, bacteria have additional mechanisms of polypeptide transport. A recently recognized Sec-independent pathway is used by E. coli and many other bacteria to secrete proteins that contain the twin-arginine motif RRX( )( ), where ( ) is a hydrophobic amino acid, in their N-terminal signal sequences. Proteins encoded by genes tatABC are required by this Tat pathway.594 598 599 Related pathways have been identified both in mitochondria and... 

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