Screening of Enzymes from Culturable Microorganisms

Screening of enzymes expressed in microorganisms, which are found in the environment and cultivated in laboratory, has the merit of finding novel enzymes because the method does not use the sequence information of already-known enzymes. However, an enzyme cannot be found if the microorganism cannot be cultured under laboratory cultivation conditions, or the microorganism does not express the enzyme under certain laboratory cultivation conditions. 

Anovel screening method using multi-well plates has been reported [4]. Microbial cultures of about 300microbes, demonstrating utility in reducing model ketones, were arrayed in multi-well plates, stored until use at -80 °C, and used to rapidly 

300 microbes on 24-deep well plate stored at -80 °C until use 

Reaction and screening, resulted in finding seven valuable oxidoreductases 

Screening of biocatalysts (a) from culturable microorganisms using multi-well plates (b) using metagenomes of complex 

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