Screening mass spectrometry-based

Both plate reader and mass spectrometry based methods are commonly used for screening. The selection of a technique depends on instrument availability, throughput needs and the stage of compound advancement. For the characterization of compounds in drug development and clinical candidates, assays carried out using drug-like probes and analyzed by LC-MS/MS methods are considered the gold standard [117]. 

Syagen Technology, Inc., Mass spectrometry based personnel screening portal, www.syagen. com/LitReq/Presl 102/portal.pdf... 

Muckenschnabel, R. Falchetto, L. M. Mayr, I. Filipuzzi SpeedScreen label-free liquid chromatography-mass spectrometry-based high-throughput screening for the discovery of orphan protein ligands. Anal. Biochem. 2004, 324, 241-249. 

Additional Affinity Screening Methodology That Includes Mass Spectrometry-based Readout 177... 

Screening for noncovalent ligand-receptor interactions by electrospray ionization mass spectrometry-based diffusion measurements. Anal. Chem. 2004, 76, 7077-7083. 

Syage, J. A., K. A. Hanold, and M. A. Hanning-Lee. Mass spectrometry based personnel screening system. Proc. INMM—42nd Annual Meeting, Palm Springs, CA, 2001. 

Femandez-Metzler, C. L., Owens, K. G., Baillie, T. A., and King, R. C. (1999). Rapid liquid chromatography with tandem mass spectrometry-based screening procedures for studies on the biotransformation of drug candidates. Drug Metab. Dispos. 27 32-40. 

Hsieh, Y., Fukuda, E., Wingate, J., and Korfmacher, W. A. (2006). Fast mass spectrometry-based methodologies for pharmaceutical analyses. Comb. Chem. High Throughput Screening 9 3-8. 

Siegel, M. M. (2005). Mass-Spectrometry-Based Drug Screening Assays for Early Phases in Drug Discovery. Wiley, Hoboken, NJ. 

As future direction, and the fact that mass spectrometry has evolved very rapidly in the last decade, other mass spectrometry based technologies such as ion mobility (Thalassinos et al., 2004 Clemmer et al., 2005) may play an important role in the screening of reactive metabolites. This separation stage is orthogonal to the LC and mass spectrometric separations and occurs on an intermediate timescale... 

K.A. Hanold, Mass Spectrometry Based Personnel Screening Portal, Proceedings of the 7th International Symposium on Analysis and Detection of Explosives, Edinburgh, Scotland, 2001. 

J.A. Syage, K.A. Hanold, and M.A. Hanning-Lee, Mass Spectrometry Based Personnel Screening System, 42nd INMM Meeting, Phoenix, AZ, 2001. 

An extension of this FTICR mass spectrometry-based screening technique has been to screen a combinatorial library for ligands to two receptors simultaneously (59, 60). In this example, the two receptors consisting of RNA constructs representing the prokaryotic (16s) rRNA and eukaryotic (18s) rRNA A-site were incubated simultaneously with an aminogly-... 

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