Screening hybridoma culture supernatants for specific antibody

9 Screening hybridoma culture supernatants for specific antibody 

The fusion cultures are examined for the growth of hybridoma colonies from seven days onwards. Mouse hybridomas tend to grow initially as discrete colonies (.sphaeroids) whereas hybridomas produced with the Y3 myeloma are quite diffuse and not easy to recognize. With fiirther growth the Y3 hybridomas form discrete colonies that can be quite firmly attached to the pla.stic. Screening is normally carried out initially at 10-14 days post-fusion by removing samples of about 200 fill of supernatant. 

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