Screening from Clone Banks

One way of alleviating a number of these problems is actually to make several parallel clone banks using different expression vectors and strains, regulatable expression systems, and low copy-number vectors. Vectors choices [28] such as plasmids vs phagemids, high vs low copy number vectors, all can affect what is cloned, and there is not one solution which will satisfy every project. Furthermore, the cloning host also makes a difference. E. coli is usually the host of choice, but Bacillus can be preferred for secretable enzymes, and yeast for eukaryotic or post-transitionally modified enzymes. 

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Vol. 68 [31]. Selection of Specific Clones from Colony Banks by Screening with Radioactive Antibody. L. Clarke, R. Hitzeman, and J. Carbon. 

The replicating virus gave rise to virus plaques that were picked 10-12 days later. Six clones were isolated from one round of plaque purification and screened for protein expression. One clone was selected, amplified on HEK 293 cells, purified by cesium chloride ultracentrifugation, and used for preparation of a Master Virus Bank (MVB). 

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