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Clone bank

Chloroplast Summary of complete and partial clone banks Many 3, 69... [Pg.164]

Clone bank Tobacco, small subclones (dicot), completely sequenced 70... [Pg.164]

Clone bank Oryza sativa (rice, monocot) completely sequenced 72... [Pg.164]

Clone bank Marchantia polymorpha (bryophyte) completely sequenced 75... [Pg.164]

Same advantages as clone banks plus All the disadvantages of... [Pg.7]

One way of alleviating a number of these problems is actually to make several parallel clone banks using different expression vectors and strains, regulatable expression systems, and low copy-number vectors. Vectors choices [28] such as plasmids vs phagemids, high vs low copy number vectors, all can affect what is cloned, and there is not one solution which will satisfy every project. Furthermore, the cloning host also makes a difference. E. coli is usually the host of choice, but Bacillus can be preferred for secretable enzymes, and yeast for eukaryotic or post-transitionally modified enzymes. [Pg.9]

There are advantages and disadvantages for each strategy. First, saving the clone banks as either DNA fibraries or transformed pools provides the most flexibihty and saves time and effort. The DNA can be transformed into any host that may be desired. Different hosts may be desired for different activities since... [Pg.10]

Clone banks Proprietary sources Source of new, undiscovered catalysts Large libraries need to be... [Pg.6]

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Banking

Banks

Screening from Clone Banks

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