Saturation diversity approach

An alternative methodology based on the ringcontent of a database, using precalculated structure-based hash codes has been proposed (110). The comparison of the hashcode tables can be used to compare two databases and the number of distinct ring-system combinations can be used as an indicator of database diversity. A method for diversity assessment called the saturation diversity approach, based on picking as many mutually dissimilar compounds as possible from a database was also proposed. The methods were used to compare a number of public databases and gave similar results. 

This method using grafting to one silicon atom of SC makes it possible to approach spatially two chemically different groups r and rt and obtain free radical structures of the (=Si-0-)2Si(r )(r) type. This leads to more diverse intermediates on the solid surface and allows one to study new chemical processes involving these intermediates, including intramolecular reactions between r and /y groups. The low-molecular radicals necessary for the first step of SC modification can conveniently be obtained from the saturated H-r molecules, and appropriate defects on the silica surface can be used as... 

Naturally, the first step of each evolutionary project is the creation of diversity. The most straightforward approach to create a library of proteins is to introduce random mutations into the gene of interest by techniques such as error-prone PCR or saturation mutagenesis. The success of random mutagenesis strategies is witnessed by their ample appearances in the different chapters of this book describing case studies of particular classes of proteins and enzymes. In addition, recombination of mutant... 

Fig. 6.34 Chemical reactions of wild-type (WT) and engineered forms of flavocytochrome P450 BM3. Examples are shown of substrates and products formed in reactions of the WT and mutant forms of P450 BM3 (BM3). The BM3 enzyme has been extensively engineered using rational, direct evolution and other approaches, and the outcomes highlight the ability of BM3 and its variants to catalyze oxidation of a wide range of chemically diverse substrates. a Hydroxylation of the supposed natural substrates for WT BM3, saturated linear chain fatty acids ( C12-...

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