Sanguinarine from Chelidonium majus

Figure 11.28 shows densitograms from the separation of Chelidonium majus quaternary alkaloids [114]. It is clearly apparent that multiple development results in better separation of the component bands. Threefold development results in separation or partial separation of eight alkaloid bands (Figure 11.28b), whereas after one run, only four zones — chelirubine, sanguinarine, chelilutine, and chelerithrine... 

From greater celandine (Chelidonium majus), widespread in Central Europe, such alkaloids as sanguinarine, cholidonine, hydrastine, berberine, and chelerythine have been isolated. Ph5rtochemical investigation of... 

Fig. 36.3 Chemical structures of sanguinarine and coptisine and CZE separation of seven alkaloids in an extract of Chelidonium majus L. Peaks (7) sanguinarine, (2) coptisine, (3) chelerythrine, (4) stylopine, (5) chelidtmme, (6) protopine, (7) allocryptopine, and (x) unknown component. Conditions 20 mM phosphate buffer, pH 3.1. Separation capillary 75 pm, i.d. uncoated fused silica, 50 cm in length (40 cm effective length). Applied voltage 16 kV. Light-emitting-diode-induced fluorescence (LED) excitation wavelength 280 nm (Modified from reference [14])...

---