Sample Prepared Under Equilibrium Conditions Prior to Spin Column Treatment

1 Sample Prepared Under Equilibrium Conditions Prior to Spin Column Treatment 

The binding of a small molecule ligand to a protein receptor follows a bimolecu-lar association reaction with second-order kinetics. For the reversible reaction of a ligand L and a protein P to form a non-covalendy bound complex C at equilibrium, Eq. (1) applies where kon and kgS represent the forward and reverse mass transfer rate constants. 

If [P]o and [L]g are the initial total protein and ligand concentrations, respectively, where  

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