Sample clean-up of biological fluids

The first MISPE protocol reported in the literature [18] involved the use of an imprinted dispersion polymer in a column format, for the selective enrichment of pentamidine (1) in urine. In this case the high selectivity of the polymer allowed the analyte to be detected directly in the eluate without the need for any further chromatographic separation (entry A in Tables 15.1 and 15.2). In view of the high selectivity of MIPs, this is a viable approach that brings the benefits of shorter analysis times and simpler instrumentation. In most cases, however, MISPE has been used prior to a chromatographic separation step. The MIP has been applied in a batch-wise extraction [23] or in columns or cartridges [20-22,24-26,33]. 

DETAILS OF THE MIP SYNTHESIS AND METHOD VALIDATION RESULTS FOR THE PROTOCOLS IN TABLE 15.1. For structures of the templates see page 361 

Polymers were prepared using MAA (A-K) or 4-vinylpyridine/MAA (1/1) (L) as functional monomers and EDMA as cross-linking monomer in the presence of the template and various solvents (porogens) as shown schematically in Fig. 5.2. (Chapter5). EtOAc = ethylacetate, THF = tetrahydrofuran, RT = room temperature. 

Molecularly imprinted polymers in solid phase extractions 

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