Saccharin separation techniques

Two main standards were developed, one for the sweeteners showing UV absorption, i.e. acesulfame, aspartame and saccharin,36 and a separate standard for cyclamate37 which does not show a substantial UV absorption. Both standards use HPLC and propose specific sample preparation procedures. HPLC is also the suitable technique for the quantitative determination of sucralose.38... 

This concept was extended to the, V-enoyl sultams 27, in which the chiral auxiliary is derived from inexpensive saccharine, but in this case the diastereoselectivity is lower than in the case of the dienophile 2079. The mixture is nevertheless separable by chromatography, and this technique can be applied to 1,3-cyclohexadiene (30) (exo/endo 1 99 d.r. 82 18) and to 2,3-dimethyl-1, 3-butadiene (d.r. 64 36)80. 

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