Rubredoxin-Oxygen Oxidoreductase and Nitric Oxide Reductases

RUBREDOXIN-OXYGEN OXIDOREDUCTASE AND NITRIC OXIDE REDUCTASES 

Another member of the family of diiron flavoproteins is the recently characterized A-type flavoprotein FprA from the anaerobic. Gram-positive acetogenic bacterium Moorella thermoacetica [607]. Similar to ROO, recombinant FprA also exists as a homodimer of 45-kDa subunits and may function both as an oxido and 

Another novel member of the family of highly conserved A-type flavoproteins is flavorubredoxin (FlRd) from E. coli [613]. It could be shown that FlRd is able to carry out reduction of O2 to water [614]. However, similar to ROO and FprA, FlRd 

The reaction mechanism of ROO has not yet been studied in detail, but based on analogy with related binuclear non-heme iron enzymes, a mechanistic scheme has been proposed whereby binding of O2 to the reduced enzyme leads to the formation of a bridging peroxo intermediate, followed by the transfer of electrons from the FMN cofactor [578]. 

MEMBRANE-BOUND DllRON PROTEINS ALTERNATIVE OXIDASE 

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