RP-HPLC retention

Valko, K., Du, C. M., Bevan, C., Reynolds, D. P., Abraham, M. H. Rapid method for the estimation of octanol/water partition coefficient (logP ,) from gradient RP-HPLC retention and a hydrogen bond addity term (EaJ ). Curr. Med. Chem. 2001, 8, 1137-1146. 

Spiked standards recovery reported, identification on aglycone RP-HPLC retention times mg/kg fresh weight Accept... 

They chose the TBDMS derivatives for reducing the polarity of hydroxyl groups of hydroxy fatty acid PNB esters because the TBDMS derivatives were resistant to hydrolysis, stable in common organic solvents, and changed the adsorption TLC and RP-HPLC retention of PNB hydroxy fatty acids more than smaller siloxy groups and because the TBDMS groups could easily be removed by fluoride ions (sodium fluoride or tetra-n-butyl ammonium fluoride) to free... 

Noteworthy, in the absence of the HR, Eq. 12 reduces to one which describes the influence of the organic modifier on RP-HPLC retention. A relationship similar to Eq. 12 can be obtained to describe the simultaneous effects of the HR stationary-phase concentration and organic modifier percentage in the eluent on analyte retention. ... 

Xp define those molecular features that govern the RP-HPLC retention of barbiturates. The topological index x decreased with increased branching of an alkyl group. The topological indices Xp Xp described the exact position of branching for the investigated barbiturates. 

However, experimental determination of logP does include correlation with such properties. For example, the ElogD method developed by Lombardo et al. [67] uses RP-HPLC retention data to determine octanol-water distribution coefficients at pH 7.4 for neutral and basic drugs in Pfizer. Moreover the authors used the same method to determine ElogD at pH 6.5, thus calculating important parameters for intestinal absorption [68],... 

SPR investigations have been carried out for a series of AAbridged compounds synthetized recently as potential antithrombotic agents. The octanol/water partition coefficient and RP-HPLC retention (log K) were determined... 

Pietrogrande MC, Kahie YD. Effect of the mobile and stationary phases on rp-hplc retention and selectivity of flavonoid compounds. J Liquid Chromatogr 1994 17 3655-3670. 

Code of branched polypeptides, based on one letter symbol of amino acids. Determined by amino acid analysis after hydrolysis n 6 M HCl at 105 for 24 h. Calculated from the number average degree of polymerisation of polylysine (/)/ ,= 61) and from the amino acid composition of branches. X=Ser/Om/Glu RP-HPLC retention time. 

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