Right-angle light scatter

Figure 2. Schematic of the SLM 8000 fluorometer. Excitation occurs through the excitation monochromator, and light emitted from the sample is observed in as many as four different positions. Photomultiplier tubes (PMTs) A, B, and C can be used to monitor fluorescence or right-angle light scatter through the monochromator (PMT A) or through filters (PUT B and C), and position D measures transmittance. Three channels can be monitored simultaneously with measurements being acquired at intervals of 1 s or less. The data are stored by the computer for subsequent manipulation.

Figure 5B. Correlation of right-angle light scatter measured by fluorometry and flow cytometry. The top panel shows flow-cytometric data of side scatter of fixed, stained cells during the time course of stimulation by 1-nM (solid line, solid circles) or 0.01-nH (dashed line, open circle) FLPEP. The bottom panel shows the corresponding right-angle light-scatter data acquired pseudo-simultaneously on live cells in the fluorometer. The flow-cytometric data have been averaged, but the fluorometry data are plotted for both duplicates from one donor. Reproduced with permission from Ref. 27. Copyright 1985 Rockefeller University Press.

The increase in Ca is initiated rapidly and begins to recover after 1 min. The order of potency correlates fairly well with the solubilities of these compounds in organic solvents (37) and their abilities to accumulate in phospholipid vesicles (38), i.e., 6>y>a>p, but not with their insecticidal activity (y 6>a p 39). At these concentrations, crystals of p-, a-, and y-HCH were evident in the cell suspensions when we made simultaneous measurements of the right-angle light scatter, indicating that the order of aqueous solubilities is 6>y>a>p. However, stimulation by 6-HCH at concentrations below its aqueous solubility limit shows a typical dose dependency of the response (Figure 10). 

Viscosimetry-right angle light-scattering detector.352... 

Right angle light scattering Population measure 0.5—40 Sampling refractive Index 52... 

PD Jager, GA DeStefano, DP McNamara. Particle-size measurement using right-angle light scattering. Pharm Technol 17 102-120, 1993. 

Fig. 2. Forward vs right-angle light-scatter profiles of granulocytes in an erythrocyte-lysed, whole-blood sample (A) and a purified granulocyte preparation (B).

Matsuzaki, K., Murase, O., Sugishita, K.,Yoneyama, S., Akada, K., Ueha, M., Nakamura, A., and Kobayashi, S. (2000), Optical characterization of liposomes by right angle light scattering and turbidity measurement, Biochim. Biophys. Acta, 31, 219-226. 

Adjust the forward and right-angle light-scatter detectors so that the granulocyte population IS clearly visible see Chapter 32, Section 3.5.3., Fig. 2). Gate on the granulocyte population, and exclude the lymphocytes and monocytes from analysis. 

M. A. Haney, C. Jackson, and W. W. Yau, SEC-viscome-try-right angle light scattering, Waters International GPC Symposium Proceedings, 1991, pp. 49-63 (www.waters.com). 

Right angle light scattering measurements of neutrophils are inversely [359]... 

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