Rhodobacter sphaeroides mutant

Woodbury, N. W., Lin, S., LIN, X. M., Peloquin, J. M., Taguchi, A. M. W., Williams, J. C., and Allen, J. P, 1995, The role of reaction-center excited-state evolution during charge separation in a Rhodobacter sphaeroides mutant with an initial electron-donor midpoint potential 260mV above wild-type. Chem. Phys., 197 4059421. 

Vos M H, Jones M R, Breton J, Lambry J-C and Martin J-L 1996 Vibrational dephasing of long- and short-lived primary donor states in mutant reaction centers ot Rhodobacter sphaeroides Biochemistry 35 2687-92... 

PHOTO-BIOLOGICAL HYDROGEN PRODUCTION BY THE UPTAKEHYDROGENASE AND PHB SYNTHASE DEFICIENT MUTANT OF RHODOBACTER SPHAEROIDES... 

A purple non-sulfur bacterium, Rhodobacter sphaeroides KD131 was isolated from nature and its mutants, Hup and /or Phb were derived by internal deletion of Hup SL and Phb C. 

L. G. Vasilieva, M. Miyake, M Hara, E. Nakada, S. Nishikata, Y. Asada, J. Miyake (1998) Characterization of a novel light-harvesting mutant of Rhodobacter sphaeroides with relation to photohydrogen producton. In Biohydrogen. (Zaborsky, ed). London. Plenum Press 123-132... 

Eraso, J.M. Kaplan, S. Oxygen-insensitive synthesis of the photosynthetic membranes of Rhodobacter sphaeroides a mutant histidine kinase. J. BacterioL, 177, 2695-2706 (1995)... 

Paddock, M.L., S.H. Rongey, E.C. Abresch, G. Feher, and M.Y. Okamura (1988). Reaction centers from three herbicide-resistant mutants of Rhodobacter-sphaeroides 2,4,1 sequence analysis and preliminary characterization. Photosyn. Res., 17 75-96. 

Wang K, Zhen Y, Sadoski R, et al. Definition of the interaction domain for cytochrome c on cytochrome c oxidase. II. Rapid kinetic analysis of electron transfer from cytochrome c to Rhodobacter sphaeroides cytochrome oxidase surface mutants. / Biol Chem 1999 274 38042-50. 

Wang K, Geren L, Zhen Y, et al. Mutants of the CuA site in cytochrome c oxidase of Rhodobacter sphaeroides II. Rapid kinetic analysis of electron transfer. Biochemistry 2002 41 2298-304. 

Chirino, A. J., Lous, E. J., Huber, M., Allen, J. P., Schenck, C. C., Paddock, M. L., Feher, G., and Rees, D. C., 1994, Crystallographic analyses of site-directed mutants of the photosynthetic reaction center from Rhodobacter sphaeroides. Biochemistry, 33 458494593. 

Fyfe, P. K., McAuley-Hecht, K. E., Ridge, J. P., Prince, S. M., Fritzsch, G., Isaacs, N. W., Cogdell, R. J., and Jones, M. R., 1998b, Crystallographic studies of mutant reaction centres from Rhodobacter sphaeroides. Photosynth. Res., 55 1339140. 

Gray, K. A., Wachtveitl, J., and Oesterhelt, D., 1992, Photochemical trapping of a bacterio-pheophytin anion in site- specific reaction-center mutants from the photosynthetic bacterium Rhodobacter sphaeroides. Eur. J. Biochem., 207 723n731. 

Mattioli, T. A., Williams, J. C., Allen, J. P, and Robert, B., 1994, Changes in primary donor hydrogen-bonding interactions in mutant reaction centers from Rhodobacter sphaeroides Identification of the vibrational frequencies of all the conjugated carbonyl groups. Biochemistry, 33 1636nl643. 

Table 2 Reaction of Ru-55-Cc with Rhodobacter Sphaeroides CcO mutants. The intracomplex rate constant A a for electron transfer from heme c to Cua, the dissociation constant K, and the second-order rate constant k2nd were at 5 mM, 45 mM, and 95 mM ionic strength, respectively, at pH 8 and 23...

A pathway of protonatable residues connects the Qb binding site with the aqueous cytoplasmic compartment. The particular residues involved in the proton transport pathway have been identified in large part using site-directed mutants. Figure 15 shows the proposed pathway of H+ transfer in Rhodobacter sphaeroides " ... 

JR Golecki, S Ventura and J Oelze (1991) The architecture of unusual membrane tubes in the B800-850 lightharvesting bacteriochlorophyll deficient mutant 19 of Rhodobacter sphaeroides. FEMS Micorbiol Lett 77 335-340... 

PJ Kiley, A Varega and S Kaplan (1988) Physiological and structural analysis of light-harvesting mutants of Rhodobacter sphaeroides. J Bacteriology 170 1103-1115... 

CN Hunter, R van Grondelle and RJ van Dorssen (1989) Construction and properties ofM2192, a mutant of Rhodobacter sphaeroides, with the B875 antenna as the sole pigment protein. Biochim Biophys Acta 973 383-389... 

McPherson, P.H., et ah. Protonation and free energy changes associated with formation of QBH2 in native and Glu-L212 — Gin mutant reaction centers from Rhodobacter sphaeroides. Biochemistry, 1994, 33, 1181-1193. 

Hughes JM, Hutter MC, Reimers JR, Hush NS. Modeling the bacterial photosynthetic reaction center. 4. The structural, electrochemical, and hydrogen-bonding properties of 22 mutants of Rhodobacter sphaeroides. J Am Chem Soc 2001 123 8550-8563. 

Fritzsch G, Ermler U, Merckel M and Michel H (1996) Crystallization and structure of the photo synthetic reaction centres from Rhodobacter sphaeroides—wild type and mutants. In Michel-Beyerle M-E (ed) Reaction Centers of Photosynthetic Bacteria. Structure and Dynamics, pp 3-13. Springer-Verlag, Berlin... 

Kugistatter A, Fritzsch G and Michel H (1999) Crystallographic analysis of the mutant reaction center M 197 (FY) from Rhodobacter sphaeroides. In Garab G (ed) Photosynthesis Mechanisms and Effects, Vol II, pp 699-702. Kluwer Academic Publishers, Dordrecht... 

Eichenberger W, Boschetti A and Michel HP (1986) Lipid and pigment composition of a chlorophyll h-deficient mutant of Chlamydomonas reinhardttii. Physiol Plant 66 589-594 Ermler U, Fritzsch G, Buchanan SK and Michel H (1994) Structure of the photosynthetic reaction centre from Rhodobacter sphaeroides at 2,65 A resolution Cofactors and protein-cofactor interactions. Structure 2 925-936 Foidl M, Golecki JR and Oelze J (1997) Phototrophic growth and chlorosome formation in Chloroflexus aurantiacus under conditions of carotenoid deficiency. Photosynth Res 54 219-226... 

---