Rhodamine-azide

Fig. 1. Schematic of the method for monitoring myristoylated and palmitoylated cellular proteins, (a) co-Alkynyl fatty acid probes for detecting protein myristoyiation and paimitoyiation. (b) (o-Alkynyl fatty acids added to growth media are metabolically incorporated into cellular fatty-acylated proteins. The cells are then lysed, Click chemistry is used to chemoselectively conjugate a biotin-azide or rhodamine-azide to fatty-acylated proteins, and the proteome is detected by western blotting or in-gel fluorescence. Alk-CI 3 and Alk-Cf4 are probes for detecting protein myristoyiation, while Alk-CI6 and Alk-CfS detect protein paimitoyiation.

Biotin-azide or rhodamine-azide 5 mM in DMSO. Aliqnot solntion and store at -20°C. Protect from light (see Note 4). 

Labeling Cell Lysates with Biotin-Azide or Rhodamine-Azide... 

The user can develop the signal by using rhodamine-azide followed by in-gel fluorescence detection. This yields minimal background and high signal-to-noise ratio compared to biotin-azide. Label cells and run gel, but replace biotin-azide with rhodamine-azide in Subheading 3.5 step 2. 

Sometimes excess free rhodamine-azide may be present and it rnns at the bottom of the gel. We find it useful to run gels until the rhodamine azide band elutes from the gel this minimizes saturation of the signal when scanning the gel. 

Sodium azide. 18,325-1,2-Azido alcohols. hydroperoxides on irrad (e.g., rhodamine B) in furnishes the azido alco Azides. Benzyli system. Other aliphatic... 

This inorganic azide has been investigated as a constituent of a binary explosive. The impact sensitivity data obtained using the Picatinny Arsenal apparatus (Figure 9) include that for barium azide made with 1-2% rhodamine B dye. 

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