Rhamnose paper chromatography

Only trace amounts of monosaccharides have so far been detected in soils. The reducing sugars in cold-water extracts of Norwegian soils amounted to less than 1% of the total soil organic matter (2% for peats). Glucose, galactose, xylose, and rhamnose have been identified by paper chromatography in sodium hydroxide extracts of various Scottish soils. 

Among the other sugars detected in the soil, only the 6-deoxyhexoses have been quantitatively determined. In Delaware soils, rhamnose and fucose, determined by quantitative paper-chromatography, amounted to 20% of the sugars. Under the conditions of furfural formation from pentoses, the 6-deoxyhexoses yield 5-methyl-2-furaldehyde this has been determined by the differential solubilities of the phloroglucides in alcohol. The proportion of 6-deoxyhexoses in some cases exceeded that of pentoses. 

Zone electrophoresis employing borate buffers is a very appropriate means to separate active polyol compounds from those species whose hydroxylgroups have been substituted. Thus mixtures of 2,4- and 3,4-di-0-methyl-L-rhamnose, which are difficult to resolve by paper chromatography, are easily separated (Table 3) (23, 24, 52). 

The compositions of the different forms of PS-60 are shown in Figure 3. Approximately 50% of native PS-60 is a car-bohydrate fraction comprised of neutral sugars and a uronic acid. The neutral sugars were identified by paper chromatography and quantitated as the aldononitrile acetate derivatives after acid hydrolysis - rhamnose and glucose were shown to be present in a molar ratio of... 

Paper and Partition Chromatography.- Ternary solvent combinations have been re-examined for the paper chromatography of sugars, with rhamnose, arabinose and galactose being used as probes. A useful mixture (EtOAc-EtOH-HaO) was promoted. ... 

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