Rhamnose biosynthetic pathway

In order to provide dTDP-deoxy sugars by combinatorial biocatalysis we have utiHzed the enzymes for the dTDP- 3-L-rhamnose pathway. The successful combination of pathway enzymes with optimized enzyme productivities (amount of product per unit of enzyme) needs a concise kinetic and inhibition analysis. Scheme 5.1 depicts the biosynthetic pathway of dTDP- 3-L-rhamnose with important km and Ki constants. The enzymes RmlA and RmlB are highly controlled by the intermediate, dTDP-4-keto-6-deoxy-a-D-glucose 3, the product 5 or by... 

Scheme 5.1 Biosynthetic pathway of dTDP-P-t-rhamnose with important kinetic and inhibitory constants [68, 70, 73], RmlA, dTDP-Gic pyrophosphorylase (EC 2.7.7.24) RmlB, dTDP-Gic 4,6-dehydratase (EC 4.2.1.46) RmlC, dTDP-4-dehydrorhamnose 3,5-epimerase (EC 5.1.3.13) RmlD, dTDP-4-dehydrorhamnose reductase (EC 1.1.1.133). An asterisk indicates data from our own analysis.

Yamase H, Zhao L, Liu H-W (2000) Engineering a hybrid sugar biosynthetic pathway production of L-rhamnose and its implication on dihydrostreptose biosynthesis. J Am Chem Soc 122 12397-12398... 

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