Sugar biosynthetic pathways

Both chemo- and bioenzymatic methods have already yielded considerable carbohydrate diversity around different skeleta. Glycosylation of novel positions and alteration of sugar biosynthetic pathways were realized for different classes of NPs. Several issues have to be solved to tap more fully into the potential of the described glycodiversification techniques. 

Yamase H, Zhao L, Liu H-W (2000) Engineering a hybrid sugar biosynthetic pathway production of L-rhamnose and its implication on dihydrostreptose biosynthesis. J Am Chem Soc 122 12397-12398... 

In order to provide dTDP-deoxy sugars by combinatorial biocatalysis we have utiHzed the enzymes for the dTDP- 3-L-rhamnose pathway. The successful combination of pathway enzymes with optimized enzyme productivities (amount of product per unit of enzyme) needs a concise kinetic and inhibition analysis. Scheme 5.1 depicts the biosynthetic pathway of dTDP- 3-L-rhamnose with important km and Ki constants. The enzymes RmlA and RmlB are highly controlled by the intermediate, dTDP-4-keto-6-deoxy-a-D-glucose 3, the product 5 or by... 

Scheme 5. Various Glycosyltransfer Reactions Related to the Biosynthesis of Glycosphingolipids having Globo-series Sugar Chains. (Broken arrows indicate the hypothetical, biosynthetic pathway.)...

All N-linked oligosaccharides include a common core of five sugars linked to Asn Mana(l,3)Mana(l,6)Man 6-(l,4)GlcNAc/3(l,4)GlcNAc/31,Asn. This reflects a common biosynthetic pathway in which sugars are added to a membrane-associated polyprenol lipid called dolichol phosphate... 

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