Rhamnose antibodies

Fig. 1.—Affinity chromatography elution pattern for anti-rhamnose antibodies in immune serum from Streptococcus mutans. The arrows indicate the point of application of the serum and of 0.5 M L-rhamnose solution. The inset is an agar-diffusion pattern of the antibodies against the antigen. (Reprinted from Carbohydrate Research, Volume 124, J. H. Pazur, M. S. Erikson, M. Tay, and P. Z. Allen, pp. 253-263, copyright 1983, with permission from Elsevier Science Ltd., The Boulevard, Langford Lane, Kidlington 0X5 1GB, UK.)...

Anti-glucosyluronate-rhamnose antibodies were isolated from the globulin fraction of serum devoid of the anti-GlcA antibodies. The latter were... 

The polysaccharide of Streptococcus pyogenes consists of rhamnose and N-acetyl glucosamine residues. Antibodies have been isolated from S. pyogenes by affinity chromatography by the technique described in an earlier section. The immune serum of S. pyogenes was provided by Dr. McCarty, Rockefeller University. The affinity pattern for the isolation of the antibodies is reproduced in Fig. (12) [45],... 

Fig. (35). Agar diflusion patterns of monoclonal antibodies with an O-antigen of N-acetyl-glucosamine and rhamnose from Shigella variant Y and potential inhibitors A, monoclonal antibodies, B, nonoclonal antibodies with N-acetyl-glucosamine, C, monoclonal antibodies with rhamnose, wells 1,2,3, and 4 antigen from Shigella at concentrations of0.25,0.05,0.01 and 0.005%.

Fig. 42.—Inhibition of monoclonal antibodies from Shigella flexerni by N-acetyl /3-D-glucos-amine (I]), and L-rhamnose (I2). (Reprinted from Journal of Immunological Methods, Volume 75, J. H. Pazur and S. A. Kelly, pp. 107-116, copyright 1984 with kind permission of Elsevier Science—NL, Sara Burgerhartstraat 25, 1055 KV Amsterdam, The Netherlands.)...

Antibodies specific for the terminal nonreducing ends as well as for internal sequences of the group-specific A variant carbohydrate of the streptococcus, a polymer of L-rhamnose with a-(l— 2) and a-(1 3) linkages, have also been found in rabbit antisera. Unlike the findings with human antidextran, the binding constants of the antibodies specific for internal sequences were of higher than those directed toward the termi-... 

---