RGS4 Protein Target

About 32 000 compounds were screened to identify compounds that bind non-covalently to RGS4 using the GPC spin column/ESI-MS methodology and 1720 compounds were identified to bind (including very weak binders) to RGS4 [15], 

The 50 highest scoring compounds in the ESI-MS analyses were each evaluated by 2D HSQC NMR in the presence of RGS4 protein (see Section 

Ratio of MS Refiponse Thru Spin Column Rexfjnnsp of—250 ne ofComnotmdatid Nonnalized Vahies 

Key Relative Mass Spec Response Nnrmutketl Mass Snee J c /Mn.se 

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A. Gilbert, K. Young Applications of GPC spin colunm/ESI-MS for identifying non-covalent inhibitors of RGS4 protein, a potential CNS target, in Proceedings of the 52nd ASMS Conference on Mass Spectrometry and Allied Topics, Nashville, 2003. 

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