RGD ligands

Peptide amphiphiles may also be used for drug delivery. For example, Marini used fluorescently labeled peptide amphiphiles that were functionalized with the cyclo-RGD ligand and found that these structures were internalized by cells (Marini et al., 2002). 

Hu DD, Batbes CE 3rd, Smith JW. An allosteric Ca2+ binding site of the b3-integrins that regulates the dissociation rate for RGD ligands. JBiolChem 1996 271 21745-21751. 

In addition to targeting tumor neovasculature, we also studied the RGD ligand targeted nanoparticle for targeting ocular neovasculature tissues [5]. The antiangiogenesis efficacy observed in ocular neovascularization mcxlels further demonstrated this... 

Todd, S. J. Scurr, D. J. Gough, J. E. Alexander, M. R. Lflijn, R. V. Enzyme-activated RGD ligands on functionahzed poly(ethylene glycol) monolayers surface analysis and ceUnlar response. Langmuir 2009, 25, 7533-7539. 

Figure 9.3 Distribution of focal adhesion proteins on RGD nanopattemed surfaces. Embryonic rat fibroblasts were seeded onto nanopattemed surfaces with either a 58- or 108-nm separation distance between RGD ligands. The localization of focal adhesion proteins vincuhn and paxillin were visualized using immunofluorescence at 3 and 24 h post-seeding. Cells seeded on the 58-nm nanopattemed surfaces showed significantly higher degrees of cell spreading compared with those on the 108-nm surfaces. Actin filaments in the fibroblasts were stained using an immunofluorescent phalloidin stain [64],...

Figure 7 Fluorescence microscopy images of 35-nm RGD-SNPs (5% RGD ligand coverage) taken np by U87 (top) and MCF7 (bottom) ceU lines. The cell nuclei were stained by DAPI the RGD-SNPs were labeled with Cy5 nnits.

Ebara, M., Yamato, M., Aoyagi, T., Kikuchi, A., Sakai, K., Okano, T. (2004). Temperature-responsive cell culture surfaces enable On-Off affinity control between cell integrins and RGDS ligands. Biomacromolecules, 5, 505-510. 

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