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Reversed-phase chromatography strategy

Polar analytes are very often a major challenge to reversed-phase chromatography. This is because even with very polar (completely aqueous) mobile phases only httle or no retention can be achieved. For these cases, there are certain strategies that are discussed in the following. [Pg.229]

Michael Hanson, Klaus K. Unger, CoUn T. Mant, and Robert S. Hodges Optimization strategies in ultrafast reversed-phase chromatography of proteins, TrAC Trends Anal. Chem., 15(2), (1996) 102-110... [Pg.64]

Zhang T, Nguyen D, Franco P. Reversed-phase screening strategies for liquid chromatography on polysaccharide-derived chiral stationary phases. J. Chromatogr. A 2010 1217 1048-1055. [Pg.1623]

Matthijs, N., Perrin, C., Maftouh, M., Massart, D.L., Vander Heyden, Y. Definition and system implementation of strategies for method development of chiral separations in normal- or reversed-phase liquid chromatography using polysaccharide-based stationary phases. 7. Chromatogr. A 2004, 1041, 119-133. [Pg.210]

A. Rapid Screening Strategies in Normal- and Reversed-Phase Liquid Chromatography... [Pg.447]

Pfeifer RF and Hill DW (1983) High-performance liquid chromatography of amino acids ion-exchange and reversed-phase strategies. Adv Chromatogr 22,37-69. [Pg.93]

Fig. 1.43 Strategies for protein identification. (A) 2D gel electrophoresis approach. (B) 2D liquid chromatography approach. lEF Isoelectric focusing, sex strong cation exchange column, RP reverse phase column, SDS-PAGE sodium dodecyl sulfate polyacrylamide gel electrophoresis. Fig. 1.43 Strategies for protein identification. (A) 2D gel electrophoresis approach. (B) 2D liquid chromatography approach. lEF Isoelectric focusing, sex strong cation exchange column, RP reverse phase column, SDS-PAGE sodium dodecyl sulfate polyacrylamide gel electrophoresis.
A strategy for the selection of the stationary phase is developed based on a discussion of the mechanism underlying the separation involved in gel filtration, reverse-phased, and ion-exchange chromatography. [Pg.13]

Since the discovery of IPC, this separation strategy has been devoted to increasing the mediocre retention of ionized samples on reversed phase stationary phases via ion-pair formation with a suitable IPR to increase the analyte hydrophobicity and, in turn, its retention. It is therefore clear that most IPC separations are performed under reversed phase conditions. Even if normal phase chromatography exploits polar... [Pg.61]

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See also in sourсe #XX -- [ Pg.197 , Pg.198 , Pg.199 , Pg.200 ]



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