Reverse sample genome probes

Shen Y, LG Stehmeier, G Voordouw (1998) Identification of hydrocarbon-degrading bacteria in soil by reverse sample genome probing. Appl Environ Microbiol 63 637-645. 

Voordouw G, JK Voordouw, RR Karkhoff-Schweiser, PM Eedorak, DWS Westlake (1991) Reverse sample genome probing, a new technique for identification of bacteria in environmental samples by DNA hybridization, and its application to the identification of sulfate-reducing bacteria in oil field samples. Appl Environ Microbiol 57 3070-3078. 

Greene, E. A. Voordouw, G. Analysis of environmental microbial communities by reverse sample genome probing. J. Microbiol. Meth. 2003,53,211-219. 

The reverse sample genome probe procedure has been used to monitor sulfate-reducing bacteria in oil-field samples (Voordouw et al. 1991), further extended to include 16 heterotrophic bacteria (Telang et al. 1997), and has been applied to evaluating the effect of nitrate on an oil-field bacterial community. 

Telang, A.J., S. Ebert, L.M. Focht, D.W. Westlake, G.E. Jenneman, D. Gevertz, and G. Voordouw. 1997. Effect of nitrate injection on the microbial community in an oil field monitored by reverse sample genome probing. Appl. Environ. Microbiol. 63 1785-1793. 

The reverse sample genome probe (RSGP) has been developed to overcome this obstacle. With RSGP, the DNA from organisms previously isolated from samples acquired from known field problems is spotted on a master filter, following which DNA isolated from a new sample is labeled with either a radioactive or fluorescent indicator and placed on the filter. Labeled DNA from the new sample sticks to the corresponding spot on the master filter when complementary strands of DNA are present. Organisms represented by the labeled spots are then known to be in the new field sample [26]. 

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