Reverse phase chromatography biogenic amines

I Krause, A Bockhardt, H Neckermann, T Henle, H Klostermeyer. Simultaneous determination of amino acids and biogenic amines by reversed-phase high-performance liquid chromatography of the dabsyl derivatives. J Chromatogr A 715 67-79, 1995. 

Romero, R., Gazquez, D., Bagur, M.G. and Sanchez-Vinas, M. (2000) Optimization of chromatographic parameters for the determination of biogenic amines in wines by reversed-phase high-performance liquid chromatography, J. Chromatogr. A, 871 (1-2), 75-83. 

Ion-pair reversed-phase liquid chromatography (LC), as introduced by Johansson et al. in 1978 (1) is still the most widely used separation technique for analysis of the biogenic amines. It is usually coupled with electrochemical detection (ECD). The use of microbore LC was introduced by Scott and Kucera in 1976 (2). These systems use columns with an internal diameter (id) of 1 mm or less. Microbore LC provides a significant advantage over conventional LC The sample injected on to a microbore column is diluted by about 20-fold less than when injected on to a normal bore (e.g., 4.6 mm id) column. In this way, the compounds of interest become easier to detect. Microbore LC offers several other advantages ... 

Another important application of IC in food industry is determination of biogenic amines. Usually, they are separated by ion-pair chromatography on a chemically bonded reversed phase, using UV detection, amperometry ", or MS detection. 

Flieger, J. Czajkowska-Zelazko, A. (2011c).Comparison of chaotropic salt and ionic liquid as mobile phase additives in reversed-phase high-performance liquid chromatography of biogenic amines. J.Sep.Sci. in press... 

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