Restriction Enzymes and Characterization of Products

More than 100 restriction endonucleases (restriction enzymes) have been isolated and purified from various bacterial species. All but a few of these enzymes recog- 

Because a particular restriction enzyme recognizes a unique sequence, the number of cuts made in a DNA molecule is limited. Depending on the size of the 

DNA probes and genes can now be synthesized by automated solid-phase methods. This is accomplished by the sequential addition of activated mono- 

Introduce plasmid with foreign DNA into host bacteria 

I Select by antibiotic resistance for cells containing recombinant DNA 

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