Big Chemical Encyclopedia

Chemical substances, components, reactions, process design ...

Articles Figures Tables About

Response of Enzyme-Based Biosensors

The theoretical description of the response of enzyme-based biosensors assumes that within the enzyme layer, steady-state concentrations of substrates and products [Pg.132]

Once substrate has arrived in the reaction layer, simple one-substrate Michaelis-Menten kinetics (Eq. 7.5 for oxidoreductase enzymes, where Eo and Er represent oxidized and reduced enzyme, respectively) yield fluxes for the formation of the enzyme-substrate complex O c) and the decomposition of this complex to form product (/p), Eqs. 7.6 and 7.7  [Pg.133]

These fluxes are simply the homogeneous reaction rates multiplied by the thickness of the enzymatic reaction layer, as given in Eq. 7.1. [Pg.133]

For amperometric transducers, it is the regeneration of Eo by reaction with an oxidant (O2 or another mediating species M2+) that results in the species actually measured by the transducer. This reaction, Eq. 7.8, results in a flux for the regeneration of Er given by Eq. 7.9  [Pg.134]

Recalling that at all distances from the transducer surface, the total enzyme concentration is equal to the sum of the concentrations of the three enzyme species ([E]T = [Eo] + [ES] + [Er]), and setting j = js = jc = jp = Je (as a direct result of the steady-state assumption), the flux j is described by Eq. 7.10  [Pg.134]


See other pages where Response of Enzyme-Based Biosensors is mentioned: [Pg.132]    [Pg.133]   


SEARCH



Biosensor enzyme

Enzyme-based biosensor

Enzyme-based biosensors

© 2024 chempedia.info