Response in Thylakoid Membranes

ELECTRICAL EVENTS AND P515 RESPONSE IN THYLAKOID MEMBRANES WIM J. VREDENBERG, OLAF VAN KOOTEN and ROBERT L.A. PETERS 

This paper aims to give a short survey of new information about the characteristics of the three distinguished reaction components I, II and III of P515, in particular in relation to the functioning of the thylakoid membrane during energization. 

Plant material (Spinach and Peperomia metallica) was obtained from laboratory cultures. Chloroplast isolation and preparation were done following procedures, described before (Schapendonk, 1980). Absorbance difference measurements were done with an instrumentation and computer-assisted processing as described elsewhere (van Kooten et al., 1983). Potential measurement in Peperomia chloroplasts by implanted micro-capillary electrodes, were also as described before (Schapendonk, 1980). 

We will give the result by presenting new data on the separate components of the P515 response in single turnover saturating light flashes. 

Reaction I, characterized by a fast (ns) rise and a single exponential 

---