Resonance assignments large proteins

Higman V, Flinders J, Hiller M et al (2009) Assigning large proteins in the solid state a MAS NMR resonance assignment strategy using selectively and extensively C-labelled proteins. J Biomol NMR 44 245-260... 

Modern Techniques in Protein NMR, ed. N. R. Krishna and L. J. Berliner, Kluwer Academic/Plenum PubL, New York, N.Y., 1998 G. M. Clore and A. M, Gronenbom, Determining Structures of Large Proteins and Protein Complexes by NMR , p. 3 K. H. Gardner and L. E. Kay, Multidimensional H-Based NMR Methods for Resonance Assignment, Structure Determination, and the Study of Protein Dynamics , p. 27... 

With the dissemination of multinuclear multidimensional NMR experiments, the automated NMR resonance assignment became feasible and indispensable, simultaneously.23,35,41,99 Typically, 10-20 individual multidimensional NMR spectra are measured for a large protein. The NMR-derived information quality definitely relies on the resonance assignment. The precious assignment has been based on and assessed by the sequential NOE continuity on the peptide sequence and consistency with the amino acid spin... 

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