Resin, extractive scintillating

Ayaz, B. and DeVol, T. A., Application of mno2 coated scintillating and extractive scintillating resins to screening for radioactivity in groundwater, Nucl. Instrum. Methods Phys. Res., Sect. A, 505, 458-161, 2003. 

Roane, J. E. and DeVol, T. A., Simultaneous separation and detection of actinides in acidic solutions using an extractive scintillating resin, Anal. Chem., 74, 5629-5634, 2002. 

TRU-Resin type materials impregnated with both extractant and scintillating fluors column sensors ... 

The standard assay contained 93 kBq [y"2P]ATP, 100 juiM Na2-ATP, 10 /xM GTP, 10 mM MgS04, 25 mM LiCl, lAA as indicated, or only its solvent DMSO (control assay), and 125 fig membrane proteins in a final volume of 500 fil buffer at pH 7.5. In experiments to look for a G-nucleotide effect on the PI turnover, GTP was replaced by GTPyS, and the hormone was omitted. The reaction was started by the addition of the membranes, incubated at room temperature, and terminated by the addition of 1 ml ice-cold stop solution containing 2-propanol/conc. HCl (100/1 v/v). The lipids were extracted from the acidified propanolic solutions with n-hexane as organic solvent [11]. After lipid extraction the inositol phosphates of the aqueous phases were separated by anion exchange on Dowex AG 1-X2 (200-400 mesh) resin [28, 29]. [ P]-label of the extracts was measured via the Cerenkov-radiation in a liquid scintillation counter. 

A method claimed to be applicable for a minimum detectable activity of less than 0.05 pCI/i of sample at the time of counting has been reported (86). Carrier was mixed with the milk sample and the mixture was heated to about 75°. Iodide was collected on an anion exchange resin (resin not specified) and then eluted as lodate by means of hypochlorite. lodate was converted to free Iodine by treatment with hydroxylamlne hydrochloride and sodium nitrite. An l2"I cycle was performed and the Iodine extracted Into toluene in which medium It was determined photometrically for chemical yield. 2-Methyl butene was added to the toluene solution and the liquid sample was mixed with a toluene-based liquid scintillation counting solution and 8-gated y coincidence counting was carried out. 

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