Residuals rhBMP

The importance of the elimination of protein adsorption to the surface to obtain good glycoform resolution should also be emphasized. This is well illustrated in the separation of rhBMP-2 mentioned earlier, where the use of a commercial hydrophilic-coated capillary with a phosphate buffer without any additive led to efficiencies in excess of 7 x 10 plates/meter and up to nine isoforms differing only in the number of mannose residues being separated (Figure 22.8) [31]. 

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