Requirements on Input Data

Requirement 1. Completeness of assignment The input chemical shift list must contain more than 90% of the nonlabile and backbone amide 1H chemical shifts. If three-dimensional or four-dimensional heteronuclear-resolved [1H,1H]-NOESY spectra are used, more than 90% of the 15N and/or 13C chemical shifts must also be available. 

Requirement 2. Self-consistency The peak lists must be faithful representations of the NOESY spectra, and the chemical shift positions of the NOESY cross peaks must be correctly calibrated to fit the chemical shift lists within the chemical shift tolerances. The range of allowed chemical shift variations ( tolerances ) for 1H should not exceed 0.02 ppm when working with homonuclear [1H,1H]-NOESY spectra, or 0.03 ppm when work- 

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