Requirements of Time-resolved Mass Spectrometry in Biocatalysis

1 Requirements of Time-resolved Mass Spectrometry in Biocatalysis 

The prerequisite for implementing MS in biochemical analysis is that the analytes are amenable to ionization using one of the available ion sources (cf. Chapter 2). A vast majority of biochemical processes occur in the condensed phase. Most enzymes are active in aqueous solutions while only a few of them can catalyze reactions in organic solvents (e.g., transesterification catalyzed by lipase [5]). Water-based buffers are not always the optimum solvents for the operation of ion sources. In fact, concentrated buffer solutions - which are often used in biochemical assays - can suppress ionization, and lead to contamination of 

Time-Resolved Mass Spectrometry From Concept to Applications, First Edition. Pawel Lukasz Urban, Yu-Chie Chen and Yi-Sheng Wang. 

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