Replication methods optical microscopy

Due to the feet, that metal ions are active, in the polymer medium they immediately appear in flic environment of the PVA molecules and form bonds with the hydroxyl groups of this polymer. Polyvinyl alcohol replicates the stmeture of the particle that it surrounds however, due to the tendency of the molecules of the metal salts or other metal compounds to combine, PVA as if envelopes the powder particles, and therefore the forms of the obtained nanostmetures can be different. The optical microscopy method allows to determine the stmeture of the nanocomposite at the early stage. 

Replication is one of the oldest methods used for the production of thin TEM specimens and it is also used for specimen preparation for optical microscopy and SEM. The procedure was first introduced by Bradley [426, 427], and it is well documented in texts on specimen preparation [428, 429]. Replicas have the surface characteristics or topography of the original specimen without the need to directly image beam sensitive materials. Reflected light microscopy of polymer surfaces is often difficult because glare from the surface limits visible detail, although... 

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