Repeated sequences specific sequence hybridization

Polyclonal antibodies can react with many epitopes, whereas MAbs are restricted to one epitope on proteins that do not have repeating sequences.24 By definition, polyclonal immunoassays are generally much more sensitive but less specific than monoclonal assays. Bispecific or hybrid antibodies can be used to increase the affinity. Bispecific antibodies are formed by the fusion of two previously established hybridomas to produce antibodies displaying the binding characteristics of both of the antibodies in one molecule.25... 

Controls. Test all your probes on normal chromosomes prepared from lymphocytes. This is essential no matter what your final application is. In addition, for interphase cytogenetics, use sections from normal tissue. Pay particular attention to specificity of hybridization. Some probes such as chromosome-specific repeat-sequence probes can bind to several chromosomes if the hybridization conditions are not correct. Use commercial probes to test your reagents. 

The different families of repeated sequences in rat DNA have been fractionated according to the thermal stability (or degree of sequence homology) of the hybrids formed by renaturation. Different fractions exhibit specificity in thermal stability and reassociation rate, but cross-hybridization between nucleotide sequences in fractions with different thermal stabilities reveals sequences common to many of them (Reed, 1969). Thus, different famUies of repeated sequences probably contain some sequences in common. 

To verify the morphogenetic hypothesis, the possibility of cross hybridization must be determined are the repeated sequences in the genomes of somatic tissues tissue-specific, i.e., can a situation exist in which in the liver, for example, genes A B C are repeated frequently, while in the thyroid genes K N M are repeated, and are these genes connected with products of the function of these organs ... 

The electrical signal of daunomycin is much more evident when hybridization occurs note that the peak area did not increase when a 21-mer sequence, noncomplementary to the immobilized oligonucleotide, was used in control experiments. The difference of the area obtained in the chronopo-tentiograms (increase compared to the immobilized ss-oligonucleotide) is reported (Fig. 5b) with each measurement repeated four times. Hybridization lasted for 6 min and the signal was observed within the 0.2-3 mgL-1 of target range. Such results show the possibility to detect specific hybridization in a short time. 

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