Relationship between Nitrogen Content and Unfermentable Reducing Substances

Total Nitrogen and Unfermentable Reducing Substances in Cane Molasses 

Molasses Sample No. Total N, % Unfermentable reducing substances expressed as invert sugar, % 

The combined nitrogen in molasses was determined before fermentation and again, in the residue, after fermentation. Table III summarizes the results. 

D-Glucose and D-fructose, when heated in a phosphate-citric acid buffer solution of pH 7 in the presence of asparagine, produce considerable amounts of unfermentable reducing substances. n-Glucose, in the 

Nitrogen in Unfermentable Portion Compared with Total Nitrogen in Cane Molasses 

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