Regulation of enzymatic activity

Hunt, C.D. 1998. Regulation of enzymatic activity. One possible role of dietary boron in higher animals and humans. Biol. Trace Elem. Res. 66 205-225. 

Regulation of enzymatic activity of catalase in the temperature range from 25 tot 40°C is demonstrated in Fig. 19.9. 

What is the advantage of such complexity in the regulation of enzymatic activity We began this chapter by stressing the central importance of catalysis to the very existence of life. The control of catalysis is also critical to life. If all possible reactions in a cell were catalyzed simultaneously, macromolecules and metabolites would quickly be broken down to much simpler chem-... 

Blomquist G. J., Tillman J. A., Reed J. R., Gu P., Vanderwel D., Choi S. and Reitz R. C. (1995) Regulation of enzymatic activity involved in sex pheromone production in the housefly, Musca domestica. Insect Biochem. Molec. Biol. 25, 751-757. 

The molecular basis for regulation of enzymatic activity through phosphorylation and dephosphorylation has been established in many enzyme systems (29). The significance of these reactions in histones, ribosomal proteins and KNA polymerase is not known. In an attempt to establish the specificity of the cyclic AMP-dependent protein kinases, the structure of several substrates have been determined (30). The data indicate that the sequence around the phosphorylated serine residue all contain two basic amino acids separated by no more than two residues from the N-terminal of the susceptible serine (e.g. -Arg-Arg-X-Y-Ser-). 

In terms of achievements, selection from libraries of phage-displayed enzymes has afforded several enzymes with modified specificities and several abzymes. A combination of in vivo selection and in vitro selection for binding to ligands unrelated to the substrates has also opened the way to the engineering of the regulation of enzymatic activity. 

Hamachi, R. Eboshi, J. Watanabe, S. Shinkai, Guest-Induced Umpolung on a Protein Sirnface A Strategy for Regulation of Enzymatic Activity , J. Am. Chem. Soc., 122,4530 (2000)... 

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