Regional assessments steps involved

The presteady-state region requires knowledge of the forward (kf) and backward (k, ) rates in the formation and reversal of enzyme-substrate complexation. Most commonly such studies involve fast-mixing techniques (e.g. stopped-flow). Of course, for very fast kf and k, processes, the ratio of kf and kf is effectively an equilibrium constant. This simplification allows the use of special relaxation techniques (e.g. temperature jump) and gives access to some very rapid steps which could not otherwise be probed. In addition, the number of component rate processes in kf or kb can be assessed using such procedures. There are several examples of enzymes which use a string of enzyme substrate complexes, as depicted in Eqn. 5. 

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