Regeneration Amino acid content

Direct Determination of the Contents of Amino Acids in Proteins. Amino acid contents of proteins are presently determined, with few exceptions, on an acid hydrolysate of the protein. In some instances, however, a particular amino acid may be at least partially destroyed by the conditions for acid hydrolyses. In other instances, a particular derivative of an amino acid of the protein may be hydrolyzed by the acid condition to regenerate the original amino acid. An analysis of such a chemically modified protein would thus need to be done on the protein before acid hydrolysis. In still other instances, there may be a need to perform a large number of analyses of different proteins for a single amino acid. The availability of a simple, direct chemical method for determination of such a single amino acid would be desirable. 

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