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Reductive amination enzyme conjugation

Conjugation of HRP by reductive amination can be done by oxidizing the carbohydrate on the enzyme and subsequently coupling to the amines on (strept)avidin (Figure 23.5). [Pg.911]

In a novel approach to enzyme stabilization, chymotrypsin, trypsin and subtilisin have been separately coupled reductive amination (NaBHjCN) to polymers prepared from N-methacrylamido-sugar monomers, themselves synthesized from 2-,3- or especially 6-aroinodeoxy-D-glucose. About 3 lysines residues per enzyme molecule are conjugated, and the conjugates are more stable than the native en mes. ... [Pg.117]

See other pages where Reductive amination enzyme conjugation is mentioned: [Pg.131]    [Pg.134]    [Pg.363]    [Pg.788]    [Pg.801]    [Pg.802]    [Pg.802]    [Pg.803]    [Pg.962]    [Pg.965]    [Pg.135]    [Pg.140]    [Pg.490]    [Pg.492]    [Pg.493]    [Pg.495]    [Pg.503]    [Pg.651]    [Pg.654]    [Pg.371]    [Pg.295]    [Pg.115]    [Pg.120]    [Pg.460]    [Pg.470]    [Pg.472]    [Pg.473]    [Pg.474]    [Pg.475]    [Pg.483]    [Pg.631]    [Pg.634]    [Pg.109]    [Pg.595]    [Pg.314]    [Pg.760]    [Pg.67]    [Pg.793]    [Pg.123]    [Pg.221]    [Pg.293]    [Pg.77]    [Pg.480]    [Pg.485]   
See also in sourсe #XX -- [ Pg.967 ]

See also in sourсe #XX -- [ Pg.636 ]

See also in sourсe #XX -- [ Pg.636 ]



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Amine conjugating

Conjugate reduction

Conjugated enzyme

Conjugates enzymes

Conjugating enzymes

Conjugation amine

Enzyme Reductive amination

Enzyme conjugation

Enzyme conjugation conjugates

Reduction enzymes

Reduction enzymic

Reductive enzymes

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