Reducing of Silver-Stained Gels

A 15% acidic fixing salt (Na2S203 5H2O) (w/v), 1.2% thiourea (w/v) in ddH20 

Solutions A and B are mixed immediately before use in a ratio given in Table 2.15. 

The weakening or destaining is performed under visual control and finished by incubation with ddH20. 

After extensive washings with ddH20 (gels with exception of remaining bands and washings are absolutely colorless), the silver staining can be repeated or a further identification method is used. 

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