Reduced Dimensionality Spectroscopy

A different set of reduced dimensionality spectra for the backbone resonance assignment was proposed by Bersch et al All experiments are of (3,2)D-type, where evolution of C , H or C nucleus is registered in the same dimension as N. The spectral analysis is described in terms of double quadrature detection in the combined dimension, where four FIDs are recorded for each time increment and the sub-spectra containing the sum and the difference of the combined chemical 

---

Szyperski T, Yeh DC, Sukumaran DK, Moseley HNB, Montelione GT (2002) Reduced dimensionality NMR spectroscopy for high-throughput protein resonance assignment. Proc Natl Acad Sci USA 99 8009-8014... 

Structure determination of soluble proteins of moderate size (up to 30 kDa) by multinuclear multidimensional NMR spectroscopy is now fairly standard with well-established protocols. In the chapter NMR with Multiple Receivers, an acquisition scheme employing multiple receivers is described which allows for faster structure determination in small molecules. Further, recently employed fast acquisition schemes such as Hadamard spectroscopy, projection-reconstruction techniques, and reduced dimensionality experiments are explained. 

Values of /CH were also determined by two-dimensional /-resolved spectroscopy using an INEPT-type pulse-sequence,29 with a BIRD pulse for the suppression of "/c H and folding in the F, dimension. Rules for the calculation of the correct values of /c H from the reduced splittings observed were given. The values were also obtained from a set of /-modulated, one-dimensional 13C spectra via the... 

The three-pulse-echo peak shift is another two-dimensional echo technique, so far applied only to electronic transitions (122,123). It integrates over r3 and keeps rx and r2 as the time variables. The data are reduced by tracing the maximum in as a function of r2, resulting in a onedimensional decay curve. Although the implementation of this type of echo spectroscopy is quite different, the essential information content is much the same as in the Raman echo approach. 

---