Redox heterologous expression

In spite of their catalytic versatility and their capacity to transform a variety of pollutant compounds, peroxidases are not applied at large scale yet. The challenges that should be solved to use peroxidases for environmental purposes have been recently reviewed [146], Three main protein engineering challenges have been identified (a) the enhancement of operational stability, specifically hydrogen peroxide stability (see Chap. 11) (b) the increase of the enzyme redox potential in order to widen the substrate range (see Chap. 4) (c) the development of heterologous expression and industrial production (see Chap. 12). 

Subramanian V, Doddapaneni H, Syed K, Yadav JS (2010) P450 redox enzymes in the white rot fungus Phanerochaete chrysosporium gene transcription, heterologous expression, and activity analysis on the purified proteins. Curr Microbiol 61 306V314... 

Ichinose H, Wariishi H (2012) Heterologous expression and mechanistic investigation of a fungal cytochrome P450 (CYP5150A2) involvement of alternative redox partners. Arch Biochem Biophys 518 8-15... 

MCO enzymes for EFC technology have eome Irom a wide variety of plant, fungal, bacterial, and even human sources. To best deseribe and understand these expression systems, the application of several published expression systems will be detailed. The majority of MCOs used for cathodie redox reaetions in EFC studies have been laceases, AOx, and BOx their heterologous expression wiU be outlined in the next seetion, with a brief summary of other MCOs sueh as eemloplasmin (CP) and bacterial MCOs. 

The functions of the above three classes of proteins are directly related to the protein-bound copper ions. In those cases where functions have been unequivocally established, they are either electron storage and transfer or redox catalysis. Representative proteins from each of the above-mentioned classes have been purified directly from their natural sources and extensively characterized both structurally and biophysically. In addition, there are well-established protocols for their expression and purification from different heterologous systems. [Factor VIII is a special case for which blue copper binding has not been experimentally demonstrated, although at least two such sites can be identified in its amino acid sequence (Section VIII).]... 

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