Recovery and amplification of DNA from antigen-selected ARM complexes

5 Recovery and amplification of DNA from antigen-selected ARM complexes 

Tabl 3 Primers for generating Ck DNA fragment for fuii-length DNA recovery by PCR 

Underlined sequences are restriction enzyme sites for Xbel (TCTAGA) and EcoRI (GAATTC) for cloning. Italicized sequence Is a (His)g tag. 

Recovery and amplifleation of genetic information frcmi antigen-selected ARM complexes 

This solution is for the first ARM cycle in the second cycle, replace primer D2 by D3. and in the third by D4. etc. 

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