Recommended Storage Refrigerator

Storage of antibodies is controversial. Hint After years of antibody use, we recommend storing antibodies in small aliquots in microfiige tubes in a -70 C freezer. These aliquots of about 10 p. contain enough liquid for one or two uses. These antibodies will last for many years at this temperature and will not dry because there is no circulating cold air. The downside is that this process requires a lot of space and a great record system to keep track of where individual antibodies are located. 

Keywords Immunohistochemistry Antibody labeling Fluorescence microscopy Fluorescent immunocytochemistry Fluorescent immunohistochemistry Indirect immunocytochemistry Immunostaining 

Sample preparation is one of the most important steps in immunocytochemistry because it generally receives the least amount of planning. Unfortunately, few researchers understand how critical the first few steps in an immunocytochemistry experiment are. In fact, the quality of the cells and tissue and the ability to get good results are totally dependent on initial fixation. To put sample preparation in perspective, perhaps the best thing to do is focus on the conclusion of the experiment -the quality of final microscopic image. Good images only come from cells and tissues that are fixed properly. 

Immunocytochemistry, DOI 10.1007/978-l-4419-1304-3 3, Springer Science-l-Business Media, LLC 2010 

All tissue and cultures for immunocytochemistry must be fixed to preserve them. Unfixed cells and tissue degenerate quickly, leaving nothing to be seen. In fact, once the tissue culture medium or blood supply is removed, the process of degeneration begins. Therefore, it is crucial that immediately upon removing the tissue from the animal or the cells from the culture medium begin the preservation process with a fixative. 

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Recommended storage time up to 1 month refrigerated storage... 

For a drug product demonstrated to be stable at 5° 3°, 2°-5°, or 2°-8°C with or without humidity control and that is intended to be stored at refrigerator temperature, the recommended storage statement for labeling may be one of the following ... 

Acids should be stored in cabinets made of plastic or wood, as described in Section 8.2.1. Reactive materials must be stored in a cool dry place out of direct sunlight. Water-reactive materials should not be stored directly under fire-sprinklers ( ) and there may be code requirements for these compounds. Some compounds, such as organic peroxides, may need to be stored under refrigerated conditions and it is imperative that these only be stored in spark-proof or explosion-proof refrigerators. Conversely, it may increase the hazard if some organic peroxides are stored at too low a temperature. Therefore, it is important to adhere to the recommended storage requirements for specific reactive chemicals. 

In general, enamines are sensitive to moisture, and this fact should be kept in mind if the enamine is not to be used immediately. If the enamine is to be stored for any length of time, refrigeration is recommended. Yellowing may occur on long storage, but this change appears to have little effect on the outcome of subsequent reactions. 

Recommendations for the design and construction of refrigerated liquefied gas storage tanks... 

International Institnte of Refrigeration, Recommendations for Chilled Storage of Perishable Produce, HR, Paris, 1979... 

The submitters recommend that the product be stored in a stoppered brown bottle in a refrigerator. Although the material can be kept at room temperature for several weeks without noticeable decomposition, gradual evolution of carbon dioxide occurs over a period of several months, with the attendant risk of explosion. However, storage in the presence of a small amount of silica gel as a drying agent extends the shelf life of the material to more than a year. 

Because of the unknown nature of this possible disappearance of parathion during refrigerated storage, it is strongly recommended that all extracts be analyzed within 48 hours after preparation. 

A five year old bottle, originally stabilised with phenothiazine, was found to have polymerised explosively in storage. The resultant black goo was not acidic, suggesting something more complicated than an acrylate polymerisation. It is recommended that this monomer be stored under refrigeration. 

The monohydrated complex exploded after storage at ambient temperature for a month. Refrigerated storage is recommended. 

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