Recombinant therapeutics

Downstream Processing of Plant-derived Recombinant Therapeutic Proteins... 

First plant-derived recombinant therapeutic protein -... 

Reichert, J. and Pavlov, A. 2004. Recombinant therapeutics - success rates, market trends and values to 2010. 

The desired gene/cDNA is normally amplified, sequenced and then introduced into an expression vector that facilitates its introduction and expression (transcription and translation) in an appropriate producer cell type. All recombinant therapeutic proteins approved to date are produced in E. coli, S. cerevisiae or in animal cell lines (mainly CHO or BHK cells). The general... 

Escherichia coli as a source of recombinant, therapeutic proteins... 

Table 5.5 Recombinant therapeutic proteins approved for general medical use that are produced in S. cerevisiae. All are subsequently discussed in the chapter indicated...

For these reasons, as well as the fact that additional tried-and-tested expression systems are already available, production of recombinant therapeutic proteins in transgenic plant systems has not as yet impacted significantly on the industry. 

Recombinant selection, 12 517 plasmid vectors for, 12 501 Recombinant technology, 11 11-14 Recombinant therapeutics... 

Dubel S. Recombinant therapeutic antibodies. Applied Microbiology and Biotechnology 74 723-729 (2007). 

Mammalian cells are commonly employed for the production of therapeutic and diagnostic proteins, since they are able to correctly synthetize the large and complex structures that the human body requires as medicine [1]. Nowadays, they are employed for the large-scale production of recombinant therapeutic proteins, monoclonal antibodies (MAbs) and viruses used in the preparation of vaccines (e.g. against rabies, hepathytis B, polio, etc) [2]. An overview of some licensed/approved products derived from mammalian cell culture is given in Table 1. 

Public Health Service Act—approves most biologies, including recombinant therapeutic proteins... 

Purification of a recombinant therapeutic protein from cell culture may involve a few simple steps. For example, affinity chromatography may be used to concentrate the protein. This can be followed with an ion-exchange step and gel filtration. 

As the latter approach has been the most common and practical approach for modifying recombinant therapeutic proteins, Amgen Inc. elected this strategy to... 

Heidemann R, Zhang C, Qi H, Rule JL, Rozales C, Park S, Chuppa S, Ray M, Michales J, Konstantinov K, Naveh D (2000), The use of peptones as medium additives for the production of a recombinant therapeutic protein in high density... 

Plant cells are another expression system, which is being evaluated for the production of recombinant therapeutic proteins. According to Hellwig... 

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