Recombinant LHCII

The immobilization of trimeric LHCII is demonstrated in Fig. 13(B). After the nickel ion activation (5), a 1 pM solution of trimeric recombinant LHCII prepared in NaP + DM buffer (20 mM sodium phosphate, pH 7.4, 0.1 % (w/v) n-dodecyl-/ -D-maltoside) was introduced into the flow cell (1). For each cycle, the protein solution was incubated in the loop for 30 min, followed by buffer rinse (2), EDTA (3), and SDS (4) regeneration. EDTA was used to competitively chelate the nickel ions and break the linkage between NTA and Histidine. SDS, as mentioned in Section 4.2, was used to detach any remaining physically adsorbed proteins. As shown in Fig. 13(B), the immobilization/regeneration cycles from (1) to (5) can be well reproduced and the baseline after every cycle stabilized at a response close to the starting level. This indicates that the... 

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