Recombinant bioluminescent Escherichia

Escherichia coli K12 TGI strain was used as a recipient for transformation. At studying of SOS-system activity the recombinant bioluminescent strain of Escherichia coli recA lux containing plasmid-borne fusions of the recA promoter-operator region to the Photorhabdus luminescens ZM 1 lux genes (GosNlIgenetika, Russia) was used. Increase of their luminescence in the presence of DNA damage factors [Rosen et al., 2000], were shown previously. Investigation of the luminescent response of this strain to UV radiation allows quantitatively estimate in a real time a SOS-system induction. 

Miyamoto, C., Byers, D., Graham, A. F., and Meighen, E. A. (1987). Expression of bioluminescence by Escherichia coli containing recombinant Vibrio harveyi DNA. ]. Bacteriol. 169 247-253. 

The enhanced chemiluminescense obtained with the horseradish peroxidase-H202-luminol (139) system was applied to the development of a CLD biosensor for p-iodophenol, coumaric acid (26), 2-naphthol and hydrogen peroxide. The enzyme was immobilized by microencapsulation in a sol-gel matrix. LOD for the phenolic compounds were 0.83 p,M, 15 nM and 48 nM, respectively. A remote version of the enhanced biosensor was designed by directly immobilizing the enzyme on the tip of an optical fiber. This model was used for H2O2 assay. LOD was 52.2 p,M, with RSD 4.7% (w = 4) °. A bioluminescent response was obtained for phenols with pA a > 7 in the presence of a recombinant Escherichia coli strain, DPD2540, containing a fabA luxCDABE fusion this behavior may have analytical applications. 

Mazul, M. M. Danilov, V. S. Tetrazolium salt effect on the bioluminescence of Escherichia coli recombinant strain. Biotekhnologiya 2002, 91-96 Chem. Abstr. 2002, 138, 68140. 

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