Recombinant adeno-associated viral virus

Samulski, R. J., Chang, L. S. and Shenk, T. (1989). Helper-free stocks of recombinant adeno-associated viruses Normal integration does not require viral gene expression. J. Virol. 63, 3822-3828. 

Wang, X. S. et al. (1998). Characterization of wild-type adeno-associated virus type 2-like particles generated during recombinant viral vector production and strategies for their elimination. J. Virol. 72, 5472-5480. 

A major drawback in the use of the traditional recombinant DNA techniques is the inability to insert foreign DNA in vivo. This problem has been addressed through the development of viral gene delivery techniques. In these approaches, viral-based vectors are used (retrovirus, adenovirus, adeno associated virus, etc.), which are able to infect targeted cells, and stably insert foreign DNA into the cells. This has tremendous advantages in that it can be used in vivo, and it is especially well suited to the correction of inborn errors of metabolism. As the safety and efficacy of these approaches are improved, it is quite likely that it will be possible to use this approach to manipulate other valuable metabolic pathways [31]. 

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